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| 优化AFLP阳性带回收克隆技术 | |
| 引用本文： | 陈亮,郭小玲,马凌波,M.S.Saghai Maroof. 优化AFLP阳性带回收克隆技术[J]. 生物技术, 2000, 0(4) |
| 作者姓名： | 陈亮 郭小玲 马凌波 M.S.Saghai Maroof |
| 作者单位： | 厦门大学生命科学学院!福建厦门361005，新乡市环境保护局!河南新乡453000，厦门大学生命科学学院!福建厦门361005，Department of Cropand Soil Environmental Science，Virginia Tech，Blacksburg!VA24061，USA |
| 摘 要： | 通过将AFLP聚丙烯酰胺变性胶切小、适当曝光过量、以聚丙烯酰胺凝胶电泳代替琼脂糖电泳检测克隆片段大小的方法 ,增加了回收克隆AFLP阳性带的准确性。该方法对于从变性和非变性大page胶上回收克隆目的片段具有普遍意义。 |
| 关 键 词： | AFLP 回收 克隆 |
Optimizing recovery and cloning of positive bands in AFLP analysis |
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| CHEN Liang ,GUO Xiao-ling ,MA Ling-bo ,M.S.Saghai Maroof. Optimizing recovery and cloning of positive bands in AFLP analysis[J]. Biotechnology, 2000, 0(4) | |
| Authors: | CHEN Liang GUO Xiao-ling MA Ling-bo M.S.Saghai Maroof |
| Affiliation: | CHEN Liang 1,GUO Xiao-ling 2,MA Ling-bo 1,M.S.Saghai Maroof 3 |
| Abstract: | The veracity of cloning positive bands in AFLP analysis was increased via cutting the large AFLP gel into a small slice,little overexposure,checking the fragment size of cloning with page replacing agarose gel electrophoresis.This method is normally suitable to recovery and cloning of positive bands from denaturing and nondenaturing page gel. |
| Keywords: | AFLP recovery clonin$ |
| 本文献已被 CNKI 等数据库收录！ | |