Partial sequence of the alpha-tubulin gene from Histomonas meleagridis isolates from the United States |
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Authors: | Gerhold Richard W Lollis Lori A McDougald Larry R Beckstead Robert B |
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Institution: | Department of Poultry Science, University of Georgia, Athens, Georgia 30602, USA. rgerhold@uga.edu |
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Abstract: | Histomonas meleagridis , the causative agent of histomoniasis, is a protozoan parasite classified in the Dientamoebidae (order Tritrichomonadida). The α-tubulin gene of 7 H. meleagridis isolates originating from either domestic chickens or turkeys from the United States was amplified by nested PCR and sequenced. A 91.4-99.8% nucleotide identity was shared among the 7 different sequences, and phylogenetic analysis disclosed that the 7 isolates were divided into at least 3 clades. These sequences had a 91-99% nucleotide identity and a 96-100% amino acid identity compared with 3 H. meleagridis α-tubulin sequences obtained from isolates originating from turkeys in Germany. Further α-tubulin gene analysis from species in the Dientamoebidae will be useful in elucidating the evolutionary relationship of these protozoans. |
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