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| 粉末X射线衍射图谱计算植物淀粉结晶度方法的探讨 | |
| 引用本文： | 徐斌,满建民,韦存虚.粉末X射线衍射图谱计算植物淀粉结晶度方法的探讨[J].植物学报,2012,47(3):278-285. |
| 作者姓名： | 徐斌 满建民 韦存虚 |
| 作者单位： | 1扬州大学教育部植物功能基因组学重点实验室/江苏省作物遗传生理重点实验室, 扬州 225009 2扬州大学测试中心, 扬州 225009 |
| 基金项目： | 国家自然科学基金(No.31071342);扬州大学科技创新培育基金(No.2011CXJ080);江苏省作物学优势学科项目 |
| 摘 要： | 植物淀粉有A-型、B-型和C-型3种晶体。以水稻(Oryza sativa)、马铃薯(Solanum tuberosum)、豌豆(Pisum sativum) 和莲藕(Nelumbo nucifera)淀粉为材料, 利用粉末X-射线衍射仪(XRD)调查了不同晶体类型淀粉的波谱特征, 探讨XRD波谱相对结晶度的计算方法。软件峰拟合法、软件曲线法、直线作图法和曲线作图法均可用于计算淀粉XRD波谱的相对结晶度, 以曲线作图法计算结果较为可靠。利用曲线作图法得出的结果表明, 稻米淀粉的结晶度与直链淀粉含量呈显著线性负相关, 酸解莲藕淀粉的结晶度与淀粉酸水解度呈显著线性正相关。酸水解使莲藕淀粉的C-型晶体转变为A-型晶体。上述研究结果为利用XRD分析植物淀粉晶体类型和计算相对结晶度提供了重要参考。 |
| 关 键 词： | 结晶度 植物淀粉 粉末X-射线衍射 |
| 收稿时间： | 2011-09-13 |
| 修稿时间： | 2012-01-17 |
Methods for Determining Relative Crystallinity of Plant Starch X-ray Powder Diffraction Spectra |
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| Bin Xu,Jianmin Man,Cunxu Wei.Methods for Determining Relative Crystallinity of Plant Starch X-ray Powder Diffraction Spectra[J].Bulletin of Botany,2012,47(3):278-285. | |
| Authors: | Bin Xu Jianmin Man Cunxu Wei |
| Institution: | 1Key Laboratories of Plant Functional Genomics of Ministry of Education and Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China; 2Testing Center, Yangzhou University, Yangzhou 225009, China |
| Abstract: | Plant starches have A-, B-, and C-type crystallinity. We investigated spectrum characteristics of starches from rice, potato, pea and lotus by X-ray powder diffraction (XRD). Peak-fitting, curved-line, straight-line mapping and curved-line mapping methods are commonly used for determining relative crystallinity of starch XRD spectra. The curved-line mapping method has reliable results. We found the relative crystallinity of rice starch negatively associated with amylose content and that of acid-modified lotus starches positively associated with acid hydrolysis degree by the curved-line mapping method. The crystallinity of lotus starch changed from C- to A-type during acid hydrolysis. These results would be useful for XRD analysis of crystallinity and relative crystallinity of plant starches. |
| Keywords: | crystallinity plant starch X-ray powder diffraction |
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