| 响应面法优化荷叶离褶伞菌丝体产漆酶条件 |
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| 引用本文： | 梁倩倩,魏生龙,席亚丽,焦扬. 响应面法优化荷叶离褶伞菌丝体产漆酶条件[J]. 菌物学报, 2016, 35(3): 335-342. DOI: 10.13346/j.mycosystema.140240 |
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| 作者姓名： | 梁倩倩 魏生龙 席亚丽 焦扬 |
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| 作者单位： | 1河西学院甘肃省应用真菌工程实验室 甘肃 张掖 7340002河西学院厅市共建甘肃省食用菌遗传育种重点实验室 甘肃 张掖 7340003河西学院农业与生物技术学院 甘肃 张掖 734000 |
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| 基金项目： | 甘肃科技厅重点实验室专项(145RTSG005)，河西学院校长基金项目(XZ2014-23)Supported by the Special Fund of Key Laboratory of Science and Technology Department of Gansu Province (145RTSG005)，Hexi University President Fund Project (XZ2014-23). |
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| 摘 要： | 为了对荷叶离褶伞产漆酶条件进行优化,在单因素实验基础上,通过最陡爬坡实验(PB)对培养基8因素进行筛选,获得影响产漆酶的3个显著性因素:葡萄糖,pH和KH2PO4;通过中心组合(CCD)设计及响应面分析确定了最优发酵条件:葡萄糖20.09g/L,酪蛋白1.5g/L,酵母提取物1.5g/L,MgSO4 3g/L,CuSO4 3.75mg/L,KH2PO4 3.97g/L,pH 4.98,VB1 0.1g/L,愈创木酚12mg/L,该条件下,漆酶酶活为829.83U/mL,较未优化对照提高46.6%.
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| 关 键 词： | 荷叶离褶伞 漆酶 响应面 |
| 收稿时间： | 2014-10-22 |
Optimization of fermentation conditions for laccase production by Lyophyllum decastes using response surface methodology |
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| Qian-Qian LIANG,Sheng-Long WEI,Ya-Li XI,Yang JIAO. Optimization of fermentation conditions for laccase production by Lyophyllum decastes using response surface methodology[J]. Mycosystema, 2016, 35(3): 335-342. DOI: 10.13346/j.mycosystema.140240 |
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| Authors: | Qian-Qian LIANG Sheng-Long WEI Ya-Li XI Yang JIAO |
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| Affiliation: | 1Gansu Engineering Laboratory of Applied Mycology of Hexi University, Zhangye, Gansu 734000, China2Gansu key Laboratory of Genetics and Breeding of Edible Fungi, Zhangye, Gansu 734000, China3College of Agriculture and Biotechnology, Hexi University, Zhangye, Gansu 734000, China |
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| Abstract: | Response surface methodology (RSM) was used to optimize the fermentation conditions of Lyophyllum decastes for laccase production. Plackett-Burman design was used to evaluate the influence of related eight factors. Three factors, playing the important roles in the medium, including glucose, pH and KH2PO4, were selected. The results of the central composite design and response surface analyses showed that the optimal levels of the main factors were: glucose 20.09g/L, casein 1.5g/L, yeast extract 1.5g/L, MgSO4 3g/L, CuSO4 3.75mg/L, KH2PO4 3.97g/L, pH 4.98, VB1 0.1g/L, and guaiacol 12mg/L. The yield of laccase produced by Lyophyllum decastes in the optimal medium reached 829.83U/mL, and increased 46.6% as compared with the yield in the initial medium. |
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| Keywords: | Lyophyllum decastes laccase response surface methodology |
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