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Biodegradation of methyl violet by Pseudomonas mendocina MCM B-402
Authors:S. Sarnaik  P. Kanekar
Affiliation:(1) Microbial Sciences Division, Agharkar Research Institute, G.G. Agarkar Road, Pune 411 004, Maharashtra, India e-mail: pradnya@aripune.ernet.in Tel.: +91-020-5654357/5653680 Fax: +91-020-5651542, IN
Abstract:Pseudomonas mendocina MCM B-402 was found to utilize a triphenylmethane dye, methyl violet as the sole source of carbon when incorporated in synthetic medium. Almost complete decolorization of methyl violet by P. mendocina was observed within 48 h of incubation at ambient temperature (28 ± 2 °C) under aerated culture conditions, when the bacteria were inoculated into Davis Mingioli's synthetic medium at a concentration of 100 mg/l medium. Methyl violet was mineralized to CO2 through three unknown intermediate metabolites and phenol. The decolorization of the dye involved demethylation. Received: 27 November 1998 / Received revision: 2 March 1999 / Accepted: 5 March 1999
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