Site-directed mutagenesis of La protease. A catalytically active serine residue. |
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| Authors: | G G Choudhury V L Sylvia A Y Sakaguchi |
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| Institution: | Shemyakin Institute of Biorganic Chemistry, USSR Academy of Sciences, Moscow. |
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| Abstract: | Comparative sequence analysis of Escherichia coli ATP-dependent La protease led to the suggestion that Ser679 is the catalytically active enzyme residue. Site-directed mutagenesis Ser679----Ala, investigation of the cells containing the mutant plasmid, and study of the partially purified mutant protein produced results in favour of this suggestion. |
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