Elaboration of the method for immunocytochemical studying cytokinin-binding protein CBP70 localization in maize seedling tissues |
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| Authors: | V S Vasil’eva A L Lushnikova L L Pavlik D A Moshkov F A Brovko |
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| Institution: | (1) Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino branch, Russian Academy of Sciences, pr. Nauki 6, Pushchino, Moscow oblast, 142290, Russia;(2) Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia |
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| Abstract: | Some approaches for the elaboration of the method for immunocytochemical detection of labile protein on sections prepared from maize (Zea mays L.) seedling tissues are outlined. The effects of severe chemical reagents applied during tissue pretreatment (fixatives, inhibitors of endogenous enzymes, solutions blocking nonspecific antibody binding) on labile protein immunoreactivity in extracts were assessed in preliminary experiments. The procedure was elaborated with a triple control, which provided for the lowest background staining on plant tissue sections and the high level of antibody specific binding. |
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| Keywords: | Zea mays cytokinin receptors immunocytochemistry monoclonal antibodies |
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