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| 硝普钠灌注对移植小肠粘膜细胞凋亡的影响 | |
| 引用本文： | 陈桂明,尹路,周光文,施敏敏,颜召文.硝普钠灌注对移植小肠粘膜细胞凋亡的影响[J].现代生物医学进展,2011,11(11):2011-2013. |
| 作者姓名： | 陈桂明 尹路 周光文 施敏敏 颜召文 |
| 作者单位： | 1. 上海交通大学附属第一人民医院普外科,上海,200080 2. 上海交通大学附属瑞金医院消化外科研究所,上海,200025 3. 上海交通大学医学院病理科,上海,200025 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 目的:探讨外源性一氧化氮(nitric oxide,NO)供体硝普钠(sodium nitroprusside,SNP)对移植小肠粘膜细胞凋亡的影响。方法:64只220～300g雄性SD大鼠随机分成3组:A1组(n=8),仅行剖腹关腹手术;A2组(n=12):12对大鼠随机作为供受体行同种异体节段小肠移植,无SNP干预;A3组(n=16):16对大鼠随机作为供受体行同种异体节段小肠移植,SNP加入灌注液进行供肠灌注。采用前述3组动物模型再灌注5小时肠造口标本,TUNEL法检测小肠蜡块标本的细胞凋亡情况。结果:与A1组(3.86±4.74%)相比,A2(22.44±10.94%)、A3组(17.12±8.44%)小肠粘膜的细胞凋亡指数均有显著增高(P<0.05),A3组较A2组细胞凋亡指数显著降低(P<0.05)。结论:小肠移植导致小肠粘膜细胞凋亡增加,外源性NO供体SNP灌注能够显著降低植入小肠的细胞凋亡,从而可能减弱粘膜屏障的损伤。 |
| 关 键 词： | 小肠移植 粘膜屏障 细胞凋亡 硝普钠 |
Effects of Sodium Nitroprusside on Mucosal Epithelium Apoptosis in Rats after Small Bowel Transplantation |
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| CHEN Gui-ming,YIN Lu,ZHOU Guang-wen,SHI Min-min,YAN Zhao-wen.Effects of Sodium Nitroprusside on Mucosal Epithelium Apoptosis in Rats after Small Bowel Transplantation[J].Progress in Modern Biomedicine,2011,11(11):2011-2013. | |
| Authors: | CHEN Gui-ming YIN Lu ZHOU Guang-wen SHI Min-min YAN Zhao-wen |
| Institution: | 1 Department of General Surgery,Jiao Tong University Affiliated First People’s Hospital,Shanghai,200080,China; 2 Institute of Digestive Surgery,Ruijin Hospital,Shanghai Jiao Tong University Medical college,Shanghai 200025,China; 3 Department of Pathology,Shanghai Jiao Tong University Medical college,Shanghai 200025,China) |
| Abstract: | Objective: To investigate the effect of sodium nitroprusside(SNP) treatment on mucosal epithelium apoptosis of the transplanted intestine in rat.Methods: Sixty-four male Sprague-Dawley(SD) rats weighing 220~300g were randomly divided into three groups.Group A1(n=8): only performed laparotomy.Group A2(n=12): 24 rats were randomly assigned to be donor or recipient to estab-lish small bowel transplantation(SBT) model,but no SNP treatment.Group A3(n=16),32 rats were randomly assigned to be donor or re-cipient t... |
| Keywords: | Small bowel transplantation Mucosal barrier Apoptosis Sodium nitroprusside |
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