炭疽受体CMG2-Fc 融合蛋白在CHO 细胞中的表达、纯化与鉴定

目的:构建炭疽受体CMG2和人IgG1 Fc片段融合基因载体,转染CHO细胞并通过毒素中和试验检测CMG2-Fc拮抗炭疽毒素(PA+LF)的能力。方法:将含有CMG2胞外区1-217AA片度基因和人IgG1的Fc片段基因共同连接入pcDNA3.1载体转染CHO细胞并筛选高表达CMG2-Fc的CHO细胞系,通过小鼠RAW264.7巨噬细胞保护试验检测CMG2-Fc拮抗炭疽毒素的能力。结果:获得了表达CMG2-Fc的细胞株,毒素中和实验显示该蛋白可以有效抑制炭疽毒素引起的细胞损伤。结论:CMG2-Fc能够保护小鼠巨噬细胞免受炭疽毒素攻击,提示其可以作为抗毒素治疗炭疽感染。

Expression,Purification and Identification of Anthrax Receptor CMG2-Fc Fusion Protein in CHO Cell

Objective: To construct fusion gene vector of anthrax receptor CMG2 and human IgG1 Fc fragent,transfect CHO cell and to testify the inhibit ability of CMG2-Fc on anthrax toxin(PA and LF) by toxin neutralization assay.Methods: An expression vector including CMG2(1-225AA) gene and Fc fragment of human immunoglobulin G(IgG) were constructed,and CHO cell line with higher CMG2-Fc expression were got.The effect of CMG2-Fc on inhibiting anthrax toxin was detected by mouse macrophage protection assay.Results: The C...