| 生半夏、南星水提物对人胃癌BGC823 细胞的侵袭力及
HIF-1αmRNA 蛋白表达的影响 |
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| 引用本文： | 毛竹君张慈安武峰魏品康.生半夏、南星水提物对人胃癌BGC823 细胞的侵袭力及
HIF-1αmRNA 蛋白表达的影响[J].现代生物医学进展,2011,11(10):1861-1864. |
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| 作者姓名： | 毛竹君张慈安武峰魏品康 |
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| 作者单位： | 第二军医大学附属长征医院中医科,上海,200003 |
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| 摘 要： | 目的:观察生半夏、南星中药水提物对缺氧环境中人胃癌细胞株BGC823细胞HIF-1α蛋白表达和侵袭力的影响。方法:运用CoCl(2氯化钴)诱导BCG823细胞缺氧,使得细胞中HIF-1α蛋白表达升高.实验组加入生半夏、南星水提物对细胞进行预处理,然后在进行缺氧诱导。通过甲基噻唑基四唑法(MTT)检测细胞活性,使用Transwell检测细胞侵袭能力变化,RT-PCR、Western blotting分别检测HIF-1α mRNA及蛋白含量及变化。结果:生半夏、南星水提物能抑制人胃癌BGC823细胞的增殖;生半夏、南星水提物均能抑制缺氧诱导胃癌细胞的侵袭力,并且能降低HIF-1α mRNA及蛋白表达。结论:生半夏、南星水提物可抑制人胃癌BGC823细胞的增殖,抑制人胃癌BGC823细胞侵袭力,可能通过降低HIF-1α蛋白表达有关。
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| 关 键 词： | 生半夏 南星 胃癌 侵袭 HIF-1α |
Effects of Aqueous Extract of Chinese Medicine Raw Pinellia and Nanxing
on Human Gastric Cancer Cells BGC823 |
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| MAO Zhu-jun,ZHANG Ci-an,WU Feng,WEI Pin-kang.Effects of Aqueous Extract of Chinese Medicine Raw Pinellia and Nanxing
on Human Gastric Cancer Cells BGC823[J].Progress in Modern Biomedicine,2011,11(10):1861-1864. |
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| Authors: | MAO Zhu-jun ZHANG Ci-an WU Feng WEI Pin-kang |
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| Institution: | △(Department of Traditional Chinese Medicine,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China) |
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| Abstract: | Objective:To investigate the effects of the enthanol and nanxing extract from Rhizome Pinelliae Preparata on hypoxia-inducible-1 alpha protein expression and aggressive influence in human gastric cancer cells BGC823.Methods:CoCl2(chlorinated cobalt) was used to induce BCG823 cytopathic hypoxia approach,which leads the increasing the expression of hypoxia-inducible-1 alpha.The cell viability was detected by MTT chromatometry and cell growth curve was generated;Transwell small room was used to detect cells at... |
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