Amino group modification inhibits ristocetin cofactor activity of human von Willebrand factor |
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Authors: | Samuel A Santoro |
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Institution: | Division of Laboratory Medicine Washington University School of Medicine St. Louis, Missouri 63110 USA |
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Abstract: | Purified von Willebrand factor rapidly loses activity when treated under mild conditions with the highly specific amino group reagent trinitrobenzenesulfonic acid. Greater than 90 percent inhibition of activity is achieved by modification of only 7 percent of the amino groups. Other modifications such as acetylation and succinylation also abolish activity. It is unlikely that the essential rapidly reacting amino groups function simply in an electrostatic manner since modifications such as amidination and methylation which produce derivatives which retain positive charge are also inactive or nearly so. |
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Keywords: | EGF epidermal growth factor Dp Diphtheria DMEM Dulbecco's modified Eagle's medium FBS fetal bovine serum |
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