Carbohydrate-free carboxypeptidase Y is transferred into the lysosome-like yeast vacuole |
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Authors: | H Schwaiger A Hasilik K von Figura A Wiemken W Tanner |
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Institution: | 1. Botanical Institute, University of Regensburg, D-8400 Regensburg, FRG;2. Institute of Physiological Chemistry, University of Münster, D-4400 Münster, FRG;3. Institute of General Botany, ETH, Zürich, Switzerland |
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Abstract: | Carboxypeptidase Y, localized in the lysosome-like yeast vacuole, has been metabolically labeled with 2-3H]mannose. After immunoprecipitation the carbohydrate moieties were released by treatment with endo-β-N-acetyl-glucosaminidase H and separated by paper electrophoresis. Evidence for the presence of phospho-monoester and -diester groups in the molecule has been obtained. In the latter phosphate links C-1 of mannose or of mannosyl 1,3-mannose to C-6 of a mannose residue within a larger oligomannose moiety. In the presence of tunicamycin yeast cells synthesize a carbohydrate-free carboxypeptidase Y, which could be traced after metabolic labeling with 14C]-phenylalanine. The carbohydrate-free enzyme was segregated into the vacuoles to the same extent as the intact glycoprotein. |
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Keywords: | Address correspondence to G L B at the Department of Anesthesiology Michael Reese Hospital and Medical Center 2900 S Ellis Ave Chicago IL 60616 |
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