A new approach (cyano-transfer) for cyanogen bromide activation of Sepharose at neutral pH,which yields activated resins,free of interfering nitrogen derivatives |
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Authors: | Joachim Kohn Meir Wilchek |
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Institution: | Department of Biophysics, The Weizmann Institute of Science, Rehovot 76100, Israel |
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Abstract: | A new approach for the reaction of Sepharose with cyanogen bromide is described, using triethylamine as a “cyano-transfer” reagent. An optimized procedure for activation at neutral pH was developed. This procedure requires only about 5% of the usual amount of cyanogen bromide. Activated resins are free of imidocarbonates and carbamates, containing only active cyanate esters. Extremely high coupling capacities (75 μmol ligand/g wet Sepharose 4B) can be obtained using this method. |
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Keywords: | TEA triethylamine DMF dimethylformamide |
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