N-terminal amino acid analysis of polypeptide chains. The use of pivalyl or benzoyl chloride as reagents for a quantitative determination by gas—liquid chromatography |
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| Authors: | J C Cavadore G Nota G Prota A Previero |
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| Institution: | 2. Groupe de Recherches U-67 (INSERM) and Centre de Recherches de Biochimie Macromoléculaire (CNRS), BP 5051, 34033-Montpellier, France.;3. Istituto di Chimica Organica dell''Università, Napoli, Italy. |
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| Abstract: | Pivalyl chloride and benzoyl chloride are utilized as reagents for the N-terminal analysis of polypeptide chains. Pivalyl and benzoyl derivatives obtained are analyzed by gas-liquid chromatography using glass capillary columns.The chromatographic resolution of the most common amino acid derivatives allows a quantitative estimation of the N-terminal residues even in the case of complicated peptide mixtures. |
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