In vitro propagation of Clerodendrum colebrookianum Walp., a potential natural anti-hypertension medicinal plant |
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| Authors: | A A Mao A Wetten M Fay P D S Caligari |
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| Institution: | (1) Department of Agricultural Botany, School of Plant Sciences, The University of Reading, Whiteknights, P.O. Box 221, RG6 2AS Reading, UK;(2) Royal Botanic Gardens, TW9 3AE Kew, Richmond, Surrey, UK |
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| Abstract: | A rapid clonal propagation system for Clerodendrum colebrookianum Walp. (Verbenaceae), a anti-hypertension folk medicinal shrub has been developed. A range of cytokinins has been investigated for multiple shoot induction with shoot apex, axillary shoot, leaf, petiole and root explants. Optimum shoot induction occurred with axillary buds using 6-benzyladenine where an average of 21 shoots were produced per explant in 6 weeks. Subculturing the newly produced shoots, by separating into groups of five shoots, produced an average of 43 new shoots per culture within 4 weeks. In vitro rooting and weaning of over 200 plantlets was completely successful. Cytological studies revealed no visible abnormalities in chromosome number.Abbreviations 2iP
2-isopentenyladenine
- BA
6-benzyladenine
- LSD
Least Significant Difference
- NAA
1-naphthaleneacetic acid
- TDZ
thidiazuron
- WPM
Woody Plant Medium (Lloyd & McCown 1980) basal medium |
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