Optimizing the production of transformed pea (Pisum sativum L.) callus using disarmed Agrobacterium tumefaciens strains |
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Authors: | Monika M Lulsdorf Hans Rempel Jennie A Jackson David S Baliski Shaun L A Hobbs |
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Institution: | (1) Plant Biotechnology Institute, NRC, 110 Gymnasium Road, S7N OW9 Saskatoon, Saskatchewan, Canada;(2) Department of Biochemistry, University of Glasgow, G12 8QQ Glasgow, UK |
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Abstract: | For optimization of the transformation procedure with Pisum sativum L. stern explant callus was used to test the effect of disarmed Agrobacterium tumefaciens strains, cocultivation procedures (preconditioning of explants; use of Nicotiana tabacum L. nurse cultures), duration of cocultivation (2, 3 or 4 days), and agents for selection (kanamycin or hygromycin). The succinamopine strain EHA101(pBI1042) produced the highest percentage of transformed calli (77%) when used in conjunction with tobacco nurse culture during four days of cocultivation. Using this strain, kanamycin (76%) and hygromycin (77%) were equally effective selective agents, but for strain LBA4404(pBI1042) percentage of transformed calli was higher for hygromycin (63%) than for kanamycin (17%). The procedures and strains shown to be optimal for transformation of pea callus will now be complemented by a pea regeneration system. |
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