Isolation and characterization of a ribonuclease III deficient mutant of Escherichia coli

Summary A mutant of E. coli K 12 AB301 RNAase ₁₉ ^- , selected for its inability to degrade double-stranded RNA, has been isolated and shown to have less than 1% of RNAase III-activity related to the parental strain.Abbreviations TCA trichloracetic acid - RF replicative form of phage-RNA Enzymes Lysozyme (E.C. 3.2.1.17) - RNAase (E.C. 2.7.7.16) - DNAase (E.C. 3.1.4.5)