Prostaglandin levels in preovulatory follicles from rabbit ovaries perfused |
| |
Authors: | Robert D Koos Martin R Clark Per O Janson Kurt EB Ahrén William J LeMaire |
| |
Institution: | 1. The Endocrine Laboratory and Department of Obstetrics & Gynecology University of Miami School of Medicine Miami, Florida 33101, USA;2. The Departments of Obstetrics & Gynecology and Physiology University of Göteborg Göteborg, Sweden |
| |
Abstract: | Prostaglandin (PG) levels in follicular fluid from preovulatory follicles of rabbit ovaries perfused were measured in order to compare PG changes in this model system with those that occur and in isolated, LH-treated follicles . One ovary from each rabbit was perfused without further treatment (control). The other ovary was exposed to LH (0.1 or 1 ug/ml) beginning 1 hour (h) after initiation of perfusion. Samples of perfusion medium were taken at frequent intervals for measurement of PGE, PGF, progesterone and estradiol 17β. The perfusions were terminated when the first ovulation occurred or appeared imminent as judged by changes in the size and shape of the follicles. Follicular fluid was then rapidly aspirated from all large follicles on both ovaries for PGE and PGF measurement.Ovulations occurred only in the LH-treated ovaries. Progesterone and estradiol levels were significantly elevated in the perfusion medium within 1 h of LH treatment in comparison to controls. PG levels in perfusion medium from the control and LH-treated ovaries were not different throughout perfusion and increased in both groups. In contrast, PG levels measured in follicular fluid from LH-treated ovaries were 4- to 5-fold greater than in fluid from control ovaries. It is concluded that ovulation induced by LH in this experimental model is accompanied by an increase in follicular PG levels similar to that seen in other and models. This difference in follicular PG levels between the LH-treated and control ovaries is, however, not reflected in the perfusion medium. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|