Molecular Detection and Complete Genome Sequences of Tomato chlorosis virus Isolates from Infectious Outbreaks in China |
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Authors: | Li‐Ming Zhao Gang Li Ying Gao Yong‐Jie Liu Guo‐Zhen Sun Xiao‐Ping Zhu |
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Institution: | State Key Laboratory of Crop Biology, College of Plant Protection, Shandong Agricultural University, , Tai'an, 271018 Shandong, China |
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Abstract: | Tomato chlorosis virus (ToCV) is a whitefly‐transmitted, phloem‐limited, bipartite Crinivirus. In 2012, severe interveinal symptoms characteristic of ToCV infections were observed in greenhouse tomato plants in the Shandong province of China. High levels of infestation by whiteflies (Bemisia tabaci), which transmit ToCV, were also observed on tomato plants in all the greenhouses investigated. The presence of ToCV was confirmed by specific RT‐PCR either in the sampled plants or in the whiteflies collected from the ventral surface of the leaves of diseased plants. The complete genomic nucleotide sequences (RNA1 and RNA2) of the Shandong isolate of ToCV (ToCV‐SDSG) were determined and analysed. ToCV‐SDSG RNA1 consisted of 8594 nucleotides encompassing four open reading frames (ORFs). ToCV‐SDSG RNA2 consisted of 8242 nucleotides encompassing nine ORFs. Phylogenetic analysis suggests that the Chinese ToCV‐SDSG isolate is most similar to the ToCV‐Florida isolate. |
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Keywords: | China
Crinivirus
tomato
Tomato chlorosis virus
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