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De novo genome sequencing and comparative genomics of date palm (Phoenix dactylifera)
Authors:Al-Dous Eman K  George Binu  Al-Mahmoud Maryam E  Al-Jaber Moneera Y  Wang Hao  Salameh Yasmeen M  Al-Azwani Eman K  Chaluvadi Srinivasa  Pontaroli Ana C  DeBarry Jeremy  Arondel Vincent  Ohlrogge John  Saie Imad J  Suliman-Elmeer Khaled M  Bennetzen Jeffrey L  Kruegger Robert R  Malek Joel A
Institution:Genomics Core, Weill Cornell Medical College in Qatar, Doha, Qatar.
Abstract:Date palm is one of the most economically important woody crops cultivated in the Middle East and North Africa and is a good candidate for improving agricultural yields in arid environments. Nonetheless, long generation times (5-8 years) and dioecy (separate male and female trees) have complicated its cultivation and genetic analysis. To address these issues, we assembled a draft genome for a Khalas variety female date palm, the first publicly available resource of its type for a member of the order Arecales. The ~380 Mb sequence, spanning mainly gene-rich regions, includes >25,000 gene models and is predicted to cover ~90% of genes and ~60% of the genome. Sequencing of eight other cultivars, including females of the Deglet Noor and Medjool varieties and their backcrossed males, identified >3.5 million polymorphic sites, including >10,000 genic copy number variations. A small subset of these polymorphisms can distinguish multiple varieties. We identified a region of the genome linked to gender and found evidence that date palm employs an XY system of gender inheritance.
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