Culturing Puccinia coronata on a Cell Monolayer of the Avena sativa Coleoptile |
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Authors: | K Mendgen Elvira Dressler |
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Institution: | Universität Konstanz, Lehrstuhl Phytopathologie der Fakultät für Biologie |
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Abstract: | A culture of Puccinia coronata on an epidermal cell monolayer dissected from the Avena sativa caleoptile is described. To induce fungal development, infection structure formation had to be induced with a heat treatment. The host cells were fed with 1 % sucrose (or glucose or manitol) to sustain fungal growth. Under these conditions, normal development of hyphae, haustoria with haustorial mother cells and uredospores occurred. Uredospores had normal infectivity. |
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