中华鳖GHRL基因SNPs的筛选及生长性状的关联分析

为探究GHRL基因多态性对中华鳖(Pelodiscus sinensis)生长性状的影响, 采用直接测序法在GHRL基因上检测到14个单核苷酸多态性位点C289T、G501T、T738C、G776T、A841G、T885C、T2960C、A2987T、G3390A、A3857C、G4718A、T4820C、A4850C、T4979C。随机选取同批繁殖的120只中华鳖用飞行时间质谱法进行SNPs位点的分型, 并分析与生长性状的相关性。检测结果显示, 所有SNP位点均符合Hardy-Weinberg平衡状态(P>0.05)。方差分析结果显示, C289T位点CT、CC基因型的5项生长数据均显著高于TT基因型(P<0.05)。S2位点AB基因型的体重、背甲长、背甲宽和裙边宽4项数据均显著高于AA基因型(P<0.05)。G3390A位点AG基因型的背甲长、背甲宽2项数据显著高于AA基因型(P<0.05)。G4718A位点AG基因型的背甲长、背甲宽、裙边宽3项数据显著高于AA基因型(P<0.05)。在GHRL基因上获得的SNP位点可能影响着中华鳖的生长性状或与之紧密连锁, 可为中华鳖分子辅助育种提供助力与参考。

SNPS DETECTION OF GHRL GENE AND ITS ASSOCIATION WITH GROWTH TRAITS OF CHINESE SOFT-SHELL TURTLE (PELODISCUS SINENSIS)

To study the association between GHRL gene and growth traits of Chinese Soft-shell Turtle (Pelodiscus sinensis), In this study, we detected and found 14 SNPs of GHRL gene of Pelodiscus Sinensis by DNA sequencing: C289T, G501T, T738C, G776T, A841G, T885C, T2960C, A2987T, G3390A, A3857C, G4718A, T4820C, A4850C and T4979C. The genotype and gene frequency of these SNPs in 120 Chinese Soft-shell Turtle were assayed by using the method of Bio-Mass Spectrometry. The result showed that all the loci were found in Hardy-Weinberg equilibrium in the Chinese Soft-shell Turtle population. The growth traits (body weight, body length, body width, body height and calipash length) of CT and CC diplotype were significantly higher than those of TT diplotype in locus C289T (P<0.05). The growth traits (Body weight, body length, body width and calipash length) of AB diplotype were significantly higher than those of AA diplotype in locus S2 (P<0.05). The growth traits (Body length and body width) of AG diplotype were significantly higher than those of AA diplotype in locus G3390A (P<0.05). The growth traits (Body length, body width and calipash length) of AG diplotype were significantly higher than those of AA diplotype in locus G4718A (P<0.05). The results suggested that those loci identified in our study were closely related to the growth performance of Chinese Soft-shell Turtle; therefore it could demonstrating great potentials to be used as a potential molecular markers for better breeding.