Process of penicillin G hydrolysis catalyzed by penicillin acylase immobilized on acylic carrier

The usefulness of penicillin acylase immobilized onto butyl acrylate — ethyl glycol dimethacrylate (called in this paper acrylic carrier) in penicillin G hydrolysis performed in a stirred tank reactor is shown. The enzyme-acrylic carrier preparation does not deteriorate its own properties in the mixing condition of slurry reactor. The experiments were carried out in a batch and a continuous stirred tank reactor as well as continuous stirred tank reactors in series. It was found to be a satisfactory agreement between experimental and predicted results. It also indicated the optimal substrate concentration range which provides the most effective enzyme operation. A superiority of the three reactors in series over the batch reactor is shown.List of Symbols C_E g/m³ equivalent enzyme concentration - C_SO mol/m³ initial penicillin G concentration - K_A mol/m³ substrate affinity constant - K_iS mol/m³ substrate inhibitory constant - K_iP mol/m³ PhAA inhibitory constant - K_iQ mol/m³ 6-APA inhibitory constant - k₃ mol/g min constant rate of dissotiation of the active complex - r mol/m³ rate of reaction - t min. reaction time - t_j min. maintenance time - degree of conversion - _B, _F dimensionless time - min. residence time - PA penicillin acylase - PG penicillin G - PhAA phenylacetic acid - 6-APA 6-aminopenicillanic acid