High-resolution comparative physical mapping of mouse Chromosome 10 in the region of homology with human Chromosome 21 |
| |
Authors: | Susan E Cole Tim Wiltshire Elizabeth E Rue Dwight Morrow Phil Hieter Christina Brahe Elizabeth M Fisher Nicholas Katsanis Roger H Reeves |
| |
Institution: | (1) Department of Physiology, 205, Johns Hopkins University School of Medicine, 725 N. Wolfe St., Baltimore, Maryland 21205, USA, US;(2) Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA, US;(3) Institute of Medical Genetics, Catholic University, Rome, Italy, IT;(4) Neurogenetics Unit, Imperial College of Medicine at St. Mary's, London, UK, GB |
| |
Abstract: | Comparative mapping of human and mouse chromosomes can be used to predict locations of homologous loci between the species,
provides the substrate to examine the process of chromosomal evolution, and facilitates the continuing development of mouse
genetic models for human disorders. A YAC contig of the region of mouse Chromosome (Chr) 10 (MMU10) that demonstrates conserved
linkage with the distal portion of human Chr 21 (HSA21) has been constructed. The contig contains all known genes mapped in
both species, defines the proximal region of homology between MMU10 and HSA22, and contains the evolutionary junction between
HSA21 and HSA22 on MMU10. It consists of 23 YACs and 2 PACs, and covers 3.2 Mb of MMU10. The average marker density for this
region is 1 marker/69 kb. Nine of 22 expressed sequences are mapped here for the first time in mouse, and two are newly characterized
expressed sequences. The contig also contains 12 simple sequence repeats (SSRs) and 16 YAC and PAC endclone markers. YAC fragmentation
analysis was used to create a physical map for the proximal 2.2 Mb of the contig. Cloning of the corresponding region of HSA21
has proven difficult, and the mouse contig includes segments absent from previously described sequence ready maps of HSA21.
Received: 22 July 1998 / Accepted: 13 November 1998 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|