Alteration of steroid hormone sensitivity during the cultivation of human mammary carcinoma cells |
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Authors: | Wolfgang E Simon Marianne Hänsel Manfred Dietel Lenchen Matthiesen Michael Albrecht Fritz Hölzel |
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Institution: | (1) Department of Obstetrics and Gynecology, University of Hamburg Medical School, Martinistrasse 52, 2000 Hamburg, West Germany;(2) Department of Pathology, University of Hamburg Medical School, Martinistrasse 52, 2000 Hamburg, West Germany |
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Abstract: | Summary During early cultivation steps of the newly derived and karyotyped human mammary carcinoma line EFM-19, the cells developed
faster growth rates and became increasingly less responsive to the presence of serum in the culture medium. No drastic alterations
of the morphology and of the karyotype were observed, and carcinoembryogenic antigen remained expressed during the course
of the cultivation. In experimental incubations at various time intervals after the explantation, the cell proliferation was
analyzed for dose-dependent effects of estradiol, cortisol, progesterone, and testosterone. After 16 wk of cultivation of
the stock culture in the presence of estradiol, the cells had acquired a distinct sensitivity to estradiol resulting in permanent
growth enhancement. The withdrawal of cortisol from the medium of the stock culture subsequently provoked the loss of the
initially noted stimulation of the proliferation by cortisol. The stimulatory effect of progesterone on the proliferation
was reversed to inhibition when the stock culture was deprived of cortisol in the growth medium. The results indicate that
the choice of steroid hormones in the stock culture medium was determining the quality of the cellular growth responses.
This work was supported by the Deutsche Forschungsgemeinschaft, SFB34. |
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Keywords: | mammary carcinoma cells karyotype steroid hormone sensitivity proliferation conditions alteration of steroid sensitivity |
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