| 人胚胎干细胞分化为神经干细胞诱导方法的对比研究 |
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| 引用本文： | 焦淑洁,许慧芳,杨华静,许杰,湛彦强,张苏明.人胚胎干细胞分化为神经干细胞诱导方法的对比研究[J].中国组织化学与细胞化学杂志,2009,18(3):233-237. |
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| 作者姓名： | 焦淑洁 许慧芳 杨华静 许杰 湛彦强 张苏明 |
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| 作者单位： | 1. 华中科技大学同济医学院附属同济医院神经内科,武汉,430030
2. 华中科技大学同济医学院附属同济医院眼科,武汉,430030 |
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| 摘 要： | 目的探讨人胚胎干细胞分化为神经干细胞过程中,经拟胚体（embryonic body,EB）法和直接分化法的不同效率。方法人胚胎干细胞常规培养消化后,分为两组：A组,经EB法分化;B组,添加noggin和ITSFn直接分化法。倒置相差显微镜观察细胞形态变化,RT-PCR检测细胞各阶段标志物,免疫荧光及流式细胞仪观察两组细胞Nestin阳性细胞率。神经干细胞继续分化,免疫荧光、RT-PCR法检测MAP2、GFAP表达。结果RT-PCR检测到OCT4、nestin表达。B组nestin阳性细胞率明显高于A组,差异有统计学意义（P〈0.01）,且诱导周期短于A组。神经干细胞继续分化,得到不同数量的神经元和胶质细胞,MAP2、GFAP分别阳性。结论在体外采用定向分化诱导,人胚胎干细胞不经EB,可直接定向分化为神经干细胞,且诱导效率比EB法高。因此直接分化法是一种经济实用的诱导方法。
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| 关 键 词： | 人胚胎干细胞 细胞分化 神经干细胞 |
DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS INTO NEURAL STEM CELLS IN VITRO |
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| Jiao Shujie,Xu Huifang,Yang Huajing,Xu Jie,Zhan Yanqiang,Zhang Suming.DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS INTO NEURAL STEM CELLS IN VITRO[J].Chinese Journal of Histochemistry and Cytochemistry,2009,18(3):233-237. |
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| Authors: | Jiao Shujie Xu Huifang Yang Huajing Xu Jie Zhan Yanqiang Zhang Suming |
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| Institution: | Jiao Shujie,Xu Huifang,Yang Huajing,Xu Jie,Zhan Yanqiang,Zhang Suming(Department of Neurology,1Department of Ophthalmology,Tongji Hospital,Huazhong University of Science and Technology,Wuhan 430030,China) |
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| Abstract: | Objective To investigate the feasibility and efficiency of differentiation of human embryonic stem cells(hESCs) into neural cells with or without a period of embryonic body(EB) culture in vitro.Methods The hESCs were digested and divided into 2 groups: group A underwent EB culture,while cells in group B were induced by noggin and ITSFn directly without EB culture.Morphologic changes were observed under inverted microscope.The different markers were detected by RT-PCR.Nestin was detected by immunofluorescenc... |
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| Keywords: | Human embryonic stem cell Cell differentiation Neural stem cell |
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