Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags |
| |
Authors: | Wang Ai-Guo Yoon Sun Young Oh Jung-Hwa Jeon Yeo-Jin Kim Mirang Kim Jeong-Min Byun Sang-Soon Yang Jin Ok Kim Joo Heon Kim Dae-Ghon Yeom Young-Il Yoo Hyang-Sook Kim Yong Sung Kim Nam-Soon |
| |
Institution: | Laboratory of Human Genomics, Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea. |
| |
Abstract: | Intrahepatic cholangiocarcinoma (ICC), a malignant tumor derived from the bile duct epithelium, is one of the leading causes of death from cancer, worldwide. However, the mechanisms related to it remain largely unknown. In this study, an analysis of the gene expression profiles for ICC was done using the frequency of the ESTs obtained from nine cDNA libraries that constructed from 4 ICC cell lines and 4 normal liver tissues. One hundred and thirty-seven genes were identified as being either up- or down-regulated in human ICC cells. Thirty genes were randomly selected to confirm their differential expression in 4 human ICC cell lines and 5 ICC tissues compared to normal liver tissues by semi-quantitative RT-PCR. Among these genes, ANXA1, ANXA2, AMBP, and SERPINC1 were further verified by immunohistochemical analyses. In conclusion, these identified genes represent potential biomarkers for ICC and represent potential targets for elucidating the molecular mechanisms that are associated with ICC. |
| |
Keywords: | Expression profiling ESTs frequency Intrahepatic cholangiocarcinoma Liver |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|