Affinity thermoprecipitation of trypsin using soybean trypsin inhibitor conjugated with a thermo-reactive polymer,poly(N-vinyl caprolactam) |
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| Authors: | Igor Yu Galaev Bo Mattiasson |
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| Institution: | (1) Department of Biotechnology, Chemical Center, Lund University, P.O. Box 124, S-221 00 Lund, Sweden |
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| Abstract: | The soybean trypsin inhibitor conjugate with a thermo-reactive water soluble polymer, poly(N-vinyl caprolactam), was successfully
used for thermally induced affinity precipitation of trypsin. The validity of the developed procedure was proven by a model
separation of trypsin from dilute solution containing a large excess of bovine serum albumin. |
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