Assessment of the cryoprotectant concentration inside a bulky organ for cryopreservation using X-ray computed tomography |
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| Institution: | 1. Departamento de Física Aplicada III, Universidad de Sevilla, Camino de los Descubrimientos s/n, 41092 Sevilla, Spain;2. Centro Nacional de Aceleradores (Universidad de Sevilla – CSIC – Junta de Andalucía), Avenida Tomas Alva Edison 7, 41092 Sevilla, Spain;1. Cancer Research Group HBP, Institut Hospital del Mar d’Investigacions Mèdiques, Barcelona, Spain;2. School of Medicine, Universitat Pompeu Fabra and UAB, Barcelona, Spain;3. Department of Surgery, Hospital del Mar, Barcelona, Spain;4. BioMIT, Electronic Engineering Department, Universitat Politècnica de València, Valencia, Spain;1. School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia;2. Aquatic Sciences, South Australian Research and Development Institute, 2 Hamra Avenue, West Beach, SA 5024, Australia;3. Department of Fisheries Biology and Genetics, Hajee Mohammad Danesh Science and Technology University, Dinajpur 5200, Bangladesh;1. Koltzov Institute of Developmental Biology of the Russian Academy of Sciences, Vavilova 26, 119334, Moscow, Russian Federation;2. Pirogov Russian National Research Medical University, Ostrovitianova 1, 117997, Moscow, Russian Federation;3. Lomonosov Moscow State University, Leninskie Gory 1, Moscow, Russian Federation |
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| Abstract: | Cryoprotection of bulky organs is crucial for their storage and for subsequent transplantation. In this work we demonstrate the capability of the X-ray computed tomography (CT) as a non-invasive method to measure the cryoprotectant (cpa) concentration inside a tissue or an organ, specifically for the case of dymethil sulfoxide (Me2SO). It is remarkable that the use of Me2SO has been leader in techniques of cells and tissues cryopreservation. Although CT technologies are mainly based in density differences, and many cpas are alcohols with densities similar to water, the use of very low energies as acceleration voltage (～70 kV) and the sulfur atom in the molecule of Me2SO makes possible the visualization of this cpa inside tissues. As result we obtain a CT signal proportional to the Me2SO concentration with a spatial resolution up to 50 μm in the case of our device. |
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| Keywords: | Organ cryopreservation Vitrification CPA concentration |
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