Inducible cyclooxygenase released prostaglandin mediates immunosuppression in acute phase of experimental Trypanosoma cruzi infection |
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| Authors: | Michelin M A Silva J S Cunha F Q C |
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| Affiliation: | Department of Biological Sciences, Immunology, Federal School of Medicine, Uberaba, MG, Brazil. michelinimuno@dcb.fmtm.br |
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| Abstract: | ![]()
We investigated the possible role of prostaglandins produced by COX-2 in the immunosuppression observed during Trypanosoma cruzi infection. Con-A-stimulated splenocytes isolated from mice on days 5, 10, and 15 of infection released large amounts of PGE2 and this release was inhibited by the treatment of animals with sodium salicylate or meloxicam. The treatment of the animals with these drugs enhanced the release of IL-2 by splenocytes from T. cruzi-infected animals and significantly reduced the blood parasitemia and delayed the mortality of the infected mice. Furthermore, the release of TNF-alpha, IFN-gamma, IL-4, and IL-10 by Con-A-stimulated splenocytes obtained from infected mice on days 5, 10, and 15 of the infection was significantly inhibited by treatment of the animals with salicylate or meloxicam. In conclusion, the results suggest that the prostaglandins produced mainly by COX-2 mediate the immunosuppression observed in the acute phase of T. cruzi infection. |
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| Keywords: | PGE2, prostaglandin E2 Con-A, concanavalin A IL, interleukin TNF-α, tumor necrosis factor α IFN-γ, interferon γ COX, cyclooxygenase Th, T helper T. cruzi, Trypanosoma cruzi Ig, immunoglobulin NO, nitric oxide MHC, major histocompatibility complex PBS, phosphate-buffered saline anti-PGE2, anti-prostaglandin E2 antibody μCi, microcuries h, hour °C, degrees celsius μg/ml, micrograms per milliliter BSA, bovine serum albumin ELISA, enzyme linked immunosorbent assay nm, nanometers HE, hematoxylin-eosin mg/kg, milligrams per kilogram ng/ml, nanograms per milliliter |
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