Iron is the intracellular metal involved in the production of DNA damage by oxygen radicals. |
| |
Authors: | A C Mello-Filho R Meneghini |
| |
Institution: | Department of Biochemistry, University of S?o Paulo, Brazil. |
| |
Abstract: | The metal chelators 1,10-phenanthroline and 2,9-dimethyl-1,10-phenanthroline (neocuproine) showed distinct abilities to prevent hydroxyl radical formation from hydrogen peroxide and Cu+ or F2(2+) (Fenton reaction) as determined by electron spin resonance. o-Phenanthroline prevented both Fe- and Cu-mediated Fenton reactions whereas neocuproine only prevented the Cu-mediated Fenton reaction. Because only 1,10-phenanthroline but not neocuproine prevented DNA strand-break formation in hydrogen peroxide-treated mammalian fibroblasts it appears that the Fe-mediated, as compared to the Cu-mediated, intranuclear Fenton reaction is responsible for DNA damage. |
| |
Keywords: | |
|
|