Assessment of the efficacy of Japanese Bacillus thuringiensis isolates against the cigarette beetle,Lasioderma serricorne (Coleoptera: Anobiidae) |
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Authors: | Tsuchiya Satoshi Kasaishi Yoshihiro Harada Haruyasu Ichimatsu Tokio Saitoh Hiroyuki Mizuki Eiichi Ohba Michio |
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Institution: | Leaf Tobacco Research Center, Japan Tobacco Inc., 1900 Idei, Oyama, Tochigi 323-0808, Japan. satoshi.tsuchiya@ims.jti.co.jp |
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Abstract: | A total of 2,652 Japanese isolates of Bacillus thuringiensis, belonging to at least 54 H serogroups, were examined for assessment of the toxicity against the cigarette beetle, Lasioderma serricorne (Coleoptera: Anobiidae). When tested with spore/parasporal inclusion mixtures, strong larvicidal activities were associated with 28 isolates (1.1%). Serologically, these toxic isolates fell into 4 known H serovars: thuringiensis (9 isolates), kurstaki (2), kenyae (2), and darmstadiensis (15). Purified parasporal inclusions of the 10 selected isolates exhibited no larvicidal activity, while the supernatants of liquid cultures showed larvicidal and/or growth inhibitory effects. The activities were fully retained after heat treatment at 100 degrees C for 10 min. Overall results suggest that beta-exotoxin (or thuringiensin)-related substances are responsible for the toxicity of the present B. thuringiensis isolates against the cigarette beetle. |
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Keywords: | Lasioderma serricorne Cigarette beetle Bacills thuringiensis Serovar Heat-stable exotoxin β-Exotoxin Thuringiensin |
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