Chromosome banding and FISH with rDNA probe in the diploid and tetraploid loach Misgurnus anguillicaudatus |
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Authors: | Ya-Juan Li Yi Tian Ming-Zhao Zhang Ping-Ping Tian Zhuo Yu Syuiti Abe Katsutoshi Arai |
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Institution: | (1) Life Science and Technology Institute, Dalian Ocean University, Dalian, 116023, People’s Republic of China;(2) Faculty of Fisheries Sciences, Hokkaido University, Hakodate Hokkaido, 041-8611, Japan; |
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Abstract: | The chromosomes of the diploid and tetraploid loach Misgurnus anguillicaudatus were analyzed by staining with Ag, chromomycin A3 (CMA3)/distamycin A (DA), and DA/4′,6-diamidino-2-phenylindole (DAPI), and using fluorescence in situ hybridization (FISH) with
5.8S + 28S rDNA as a probe. Nucleolus organizer regions (NORs) were mapped to the telomeric region of the short arms of the
largest (first) metacentric chromosome pair in the diploid loach with 2n = 50 and the homologous quartet in the tetraploid loach with 4n = 100. The NORs were positive at the same region of the first metacentric chromosome for Ag and CMA3/DA stainings, but negative for DA/DAPI staining. Four signals at the homologs within the same quartet suggest the duplication
of the entire genome from diploid to tetraploid status. However, a size difference was detected between the rDNA signals by
FISH and CMA3 banding. |
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