化橘红黄酮类化合物抑制亚硝化反应活性研究

根据超声波最佳提取工艺条件提取得到化橘红总黄酮，经乙酸乙酯萃取分为酯溶性和水溶性两部分，比较它们对亚硝化反应的抑制作用，并分别采用硅胶柱层析对酯溶性成分分离纯化，采用大孔吸附树脂法对水溶性成分进行分离纯化，得到4个主要的黄酮类化合物，研究它们对亚硝化反应的抑制作用，以期得到抑制亚硝化活性较强的化合物。结果表明化橘红总黄酮提取率可达26.42%，酯溶性和水溶性部分均能阻断亚硝胺的合成及清除亚硝酸盐，其中水溶性黄酮提取物作用较强，分离得到的4个黄酮类化合物中柚皮苷的抑制作用较强，对亚硝胺的合成的最大阻断率可达94.7%，对亚硝酸盐的最大清除率可达92.3%。

Inhibition Effects of Flavonoids from Exocarpium citri Grandis on Nitrosation

The total flavonoids in Exocarpium citri Grandis were extracted using the optimum ultrasonic extraction conditions and were further separated by ethyl acetate to yield lipid-soluble and water-soluble parts. The inhibitions of these different extracts on nitrosation were studied. The lipid-soluble extract was purified by chromatography on silica gel and the water-soluble extract was purified by macroporous adsorption resin. The structures of the flavonoids were identified and their inhibitions on nitrosation were experimented. The results showed that the extraction yield of the flavonoids was as high as 26.42%. The lipid-soluble and water-soluble extracts had some inhibition effects on nitrosation such as nitrosodimethylamine synthesis and sodium nitrite, in which the inhibition of the water-soluble extract was stronger than the lipid-soluble extract. In the four flavonoids, naringin had stronger inhibition than the others, the maximum blocking rate to nitrosodimethylamine synthesis was as high as 94.7%, while the highest removal rate to sodium nitrite was 92.3%.