酶的分子设计、改造与工程应用

酶工程的研究已经发展到分子水平 ,在体外通过基因工程、化学、物理等手段改造酶分子结构与功能 ,大幅提高了酶分子的进化效率和催化效率 ,生产有价值的非天然酶。对酶工程学若干“热点”和前沿课题的研究、应用进行了概述 ,分析了国际上酶工程研究及应用技术、手段、方法 ,包括体外分子进化、核酶和抗体酶的设计、酶分子的定向固定化技术、酶蛋白分子的化学修饰、融合酶、人工合成及模拟酶等技术 ,并展望了酶工程的技术进步和应用的新进展。

Current Researches and Applications of the Molecular Enzyme Engineering

In vitro enzyme structure and function can be reconstructed in molecule level by means of genetic engineering as well as method of chemic and physics to improve enzyme efficiency and make valuable enzyme for industry application.Recent researches together with development and application advances of the molecular enzyme engineering are reviewed and discussed in this article including the technology of invitro molecular directed evolution,designation of ribozyme,abzyme,enzyme sited immobilization,fusion enzyme and synzymes etc.Further more the progress and application of enzyme engineering are also prospected.;