The first crenarchaeon capable of growth by anaerobic carbon monoxide oxidation coupled with H2 production |
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| Institution: | 1. Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia;2. Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia;3. Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia;1. Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA;2. BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge TN 37830, USA;3. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA;1. Faculty of Engineering Technology, Universiti Malaysia Pahang, 26300 Gambang, Malaysia;2. Department of Petroleum and Mining Engineering, Jashore University of Science and Technology, Jashore, 7408, Bangladesh;3. Department of Chemical Engineering and Polymer Science, Shahjalal University of Science and Technology, Sylhet, 3114, Bangladesh;4. Bioenvironmental Engineering Research Unit (BERC), Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Kuala Lumpur, Malaysia;1. Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea;2. Department of Environmental Engineering, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea;1. Department of Petroleum and Mining Engineering, Jashore University of Science and Technology, Jashore, 7408, Bangladesh;2. Faculty of Civil Engineering Technology, Universiti Malaysia Pahang, 26300, Gambang, Malaysia;3. Earth Resources & Sustainability Center, Universiti Malaysia Pahang, 26300, Gambang, Malaysia;4. Department of Chemical Engineering and Polymer Science, Shahjalal University of Science and Technology, Sylhet, 3114, Bangladesh |
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| Abstract: | The ability to grow by anaerobic CO oxidation with production of H2 from water is known for some thermophilic bacteria, most of which belong to Firmicutes, as well as for a few hyperthermophilic Euryarchaeota isolated from deep-sea hydrothermal habitats. A hyperthermophilic, neutrophilic, anaerobic filamentous archaeon strain 1505 = VKM B-3180 = KCTC 15798 was isolated from a terrestrial hot spring in Kamchatka (Russia) in the presence of 30% CO in the gas phase. Strain 1505 could grow lithotrophically using carbon monoxide as the energy source with the production of hydrogen according to the equation CO + H2O → CO2 + H2; mixotrophically on CO plus glucose; and organotrophically on peptone, yeast extract, glucose, sucrose, or Avicel. The genome of strain 1505 was sequenced and assembled into a single chromosome. Based on 16S rRNA gene sequence analysis and in silico genome-genome hybridization, this organism was shown to be closely related to the Thermofilum adornatum species. In the genome of Thermofilum sp. strain 1505, a gene cluster (TCARB_0867-TCARB_0879) was found that included genes of anaerobic (Ni,Fe-containing) carbon monoxide dehydrogenase and genes of energy-converting hydrogenase (Ni,Fe]-CODH–ECH gene cluster). Compared to the Ni,Fe]-CODH–ECH gene clusters occurring in the sequenced genomes of other H2-producing carboxydotrophs, the Ni,Fe]-CODH–ECH gene cluster of Thermofilum sp. strain 1505 presented a novel type of gene organization. The results of the study provided the first evidence of anaerobic CO oxidation coupled with H2 production performed by a crenarchaeon, as well as the first documented case of lithotrophic growth of a Thermofilaceae representative. |
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| Keywords: | Carbon monoxide Hydrogenogenic carboxydotroph Carbon monoxide dehydrogenase Energy-converting hydrogenase |
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