Detection of a novel Chlamydia species in captive spur-thighed tortoises (Testudo graeca) in southeastern Spain and proposal of Candidatus Chlamydia testudinis |
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| Institution: | 1. University Paris-Est, ANSES, Animal Health Laboratory, Bacterial Zoonoses Unit. Maisons-Alfort, France;2. Departamento de Sanidad Animal, Facultad de Veterinaria, Campus Regional de Excelencia internacional, Campus Mare Nostrum, Universidad de Murcia, Spain;3. Department of Cattle and Sheep Diseases/Laboratory of Serological Diagnosis, National Veterinary Research Institute, Pulawy, Poland;4. Friedrich-Schiller-Universität Jena, Institut f. Bioinformatik, 07743 Jena, Germany;1. State Veterinary Institute Prague, Prague, Czechia;2. University of Veterinary and Pharmaceutical Sciences, Department of Biology and Wildlife Diseases, Brno, Czechia;1. Burwood Bird and Animal Hospital, 128 Highbury Rd, Burwood, Vic 3125, Australia;2. Department of Physiology, Anatomy and Microbiology, School of Life Sciences, La Trobe University, Bundoora, Vic 3086, Australia;3. Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, Vic 3052, Australia;4. Greencross Vets Springvale, 570 Springvale Rd, Springvale South, Vic 3172, Australia;5. Wildlife Health Victoria: Surveillance, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Werribee, Vic 3030, Australia;6. School of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW 2678, Australia;1. DIMES, Microbiology, Policlinico S. Orsola, University of Bologna, Italy;2. Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Switzerland;3. National Reference Laboratory for Animal Chlamydioses, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (IZSLER), Pavia, Italy;1. University Paris-Est, ANSES, Animal Health Laboratory, Bacterial Zoonoses Unit, Maisons-Alfort, France;2. Laboratoire de l’environnement et de l’alimentation de la Vendée, La Roche sur Yon, France;3. Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian, EH26 0PZ, United Kingdom;4. Cavac, La Roche sur Yon, France;1. University Paris-Est, Anses, Animal Health Laboratory, Bacterial Zoonoses Unit, Maisons-Alfort, France;2. Parc Zoologique de Paris, Muséum National d''Histoire Naturelle, 53 Avenue de Saint-Maurice, Paris, France;3. Vébio, Arcueil, France;4. IHP VETO, Nantes, France;5. Institute of Veterinary Pathology, University of Zurich, Zurich, Switzerland;1. Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, United Kingdom;2. Bacterial Zoonoses Unit, French Agency for Food, Environmental & Occupational Health Safety (Anses), Maisons-Alfort, France;3. Friedrich-Loeffler-Institute (Federal Research Institute for Animal Health), Institute of Molecular Pathogenesis, Jena, Germany;4. Infection Genomics, Wellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom |
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| Abstract: | The spur-thighed tortoise (Testudo graeca) is an endangered Mediterranean tortoise that lives in North Africa, Southern Europe and Southwest Asia. In the wake of recent legislation making their keeping as domestic animals illegal, many of these animals have been returned to wildlife recovery centers in Spain. In the present study, a population of such tortoises showing signs of ocular disease and nasal discharge was examined for the presence of Chlamydia spp. Cloacal, conjunctival and/or choanal swabs were collected from 58 animals. Using a real-time PCR specific for the family Chlamydiaceae, 57/58 animals tested positive in at least one sample. While only a few samples proved positive for C. pecorum, sequencing of the 16S rRNA gene revealed a sequence identical to previously published sequences from specimens of German and Polish tortoises. Whole-genome sequences obtained from two conjunctival swab samples, as well as ANIb, TETRA values and a scheme based on 9 taxonomic marker genes revealed that the strain present in the Spanish tortoises represented a new yet non-classified species, with C. pecorum being its closest relative. We propose to designate the new species Candidatus Chlamydia testudinis. |
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| Keywords: | WGS Real-time PCR |
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