Cloning theClostridium thermocellum thermostable laminarinase gene inEscherichia coli: The properties of the enzyme thus produced |
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Authors: | V V Zverlov G A Velikodvorskaya |
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Institution: | (1) Institute of Molecular Genetics, USSR Academy of Sciences, Kurchatov sq. 16, 123182 Moscow, USSR |
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Abstract: | Summary We have cloned a 1.9-kb-long fragment ofClostridium thermocellum DNA which encodes laminarinase (EC 3.2.1.39). The enzyme hydrolyzes the -1,3-glucoside bonds in -1,3-and in mixed -1,3-1,4-polyglucans. The enzyme's optimum pH value is around 8.5, temperature optimum –70°C. PAGE-determined mol. weight –32 kDa.Abbreviations used CMC
carboxymethyl cellulose
- pNPC
p-nitrophenyl D cellobioside
- pNPLac
p-nitrophenyl- D-lactoside
- pNPG
p-nitrophenyl D glucopyranoside
- pNPGal
p-nitrophenyl- D galactopyranoside
- pNPXyl
p-nitrophenyl-
- D
xylopyranoside
- Ap
ampicillin
- SDS-PAGE
SDS polyacrylamide gel electrophoresis |
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Keywords: | |
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