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PINK1/Parkin-mediated mitophagy enhances the survival of Staphylococcus aureus in bovine macrophages |
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| Authors: | Xi Zhou Kangjun Liu Jianji Li Luying Cui Junsheng Dong Jun Li Xia Meng Guoqiang Zhu Heng Wang |
| Institution: | 1. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China;2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China
Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou, China Contribution: Formal analysis (equal), Investigation (equal), Methodology (equal), Software (equal);3. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou, China Contribution: Conceptualization (supporting), Methodology (supporting);4. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou, China Contribution: Methodology (supporting);5. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou, China Contribution: Software (supporting) |
| Abstract: | Mitochondria are cellular organelles that are involved in various metabolic processes, and damage to mitochondria can affect cell health and even lead to disease. Mitophagy is a mechanism by which cells selectively wrap and degrade damaged mitochondria to maintain cell homeostasis. However, studies have not focused on whether mitophagy is involved in the occurrence of Staphylococcus aureus (S. aureus)-induced mastitis in dairy cows. Here, we found that S. aureus infection of bovine macrophages leads to oxidative damage and mitochondria damage. The expression of LC3, PINK1 and Parkin was significantly increased after intracellular infection. We observed changes in the morphology of mitochondria and the emergence of mitochondrial autolysosomes in bovine macrophages by transmission electron microscopy and found that enhanced mitophagy promoted bacterial proliferation in the cell. In conclusion, this study demonstrates that S. aureus infection of bovine macrophages induces mitophagy through the PINK1/Parkin pathway, and this mechanism is used by the bacteria to avoid macrophage-induced death. These findings provide new ideas and references for the prevention and treatment of S. aureus infection. |
| Keywords: | bovine macrophages mitophagy PINK1 Staphylococcus aureus |