High density microcarrier culture with a new device which allows oxygenation and perfusion of microcarrier cultures |
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| Authors: | M Reiter F Weigang W Ernst H W D Katinger |
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| Institution: | (1) Institute of Applied Microbiology, University of Agriculture and Forestry, 1190 Vienna, Austria |
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| Abstract: | A novel system useful for aeration and cell retention in continuous perfused microcarrier cultures is described. The system is based on a vibrating cage that separates cells and microcarriers from the oxygenation chamber and allows gas bubble free oxygen transfer. In the cultivation of monkey kidney cells (VERO) on gelatin coated microcarriers, using different concentrations (5, 10 and 15 g Cytodex 3/liter) cell densities up to 107 cells per ml were obtained. The described system is scaleable. |
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| Keywords: | microcarrier culture oxygenation immobilisation perfusion |
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