Isolation and characterization of Pseudomonas putida R-prime plasmids |
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| Authors: | R Bray D Strom J Barton H F Dean A F Morgan |
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| Institution: | Department of Genetics, Monash University, Clayton, Victoria, Australia. |
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| Abstract: | A number of enhanced chromosome mobilizing (ECM) plasmids derived from the wide host range plasmid R68 have been used to construct R-prime plasmids carrying a maximum of two map minutes of the Pseudomonas putida PPN chromosome, using Pseudomonas aeruginosa PAO as the recipient. For one ECM plasmid, pMO61, the ability to form R-primes did not correlate with the ability to mobilize chromosomes in intrastrain crosses, suggesting that different mechanisms are involved. Physical analysis of one R-prime showed that 3.5 kb of chromosomal DNA had been inserted between the tandem IS21 sequences carried by the parent ECM plasmid. |
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