Liquid overlaying improves somatic embryogenesis in <Emphasis Type="Italic">Catharanthus roseus</Emphasis> |
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Authors: | Zahid H Siddiqui A Mujib Mehpara Maqsood |
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Institution: | (1) Cellular Differentiation and Molecular Genetics Section, Department of Botany, Hamdard University, New Delhi, 110062, India; |
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Abstract: | Somatic embryos were induced from in vitro germinating seed-hypocotyls of Catharanthus roseus. The process of embryogenesis has been categorized into a few distinct stages (induction, proliferation, maturation and germination)
in which liquid overlaying at varying levels 0 ml (T0), 0.25 ml (T1), 0.5 ml (T2), 0.75 ml (T3) and 1.0 ml (T4) was applied on solid medium. It was found that liquid overlaying improved proliferation; maturation, germination of embryos
in C. roseus. In proliferation stage, particularly in T2, torpedo embryo number increased significantly (i.e. 129.6%) as compared to control. Liquid overlaying (T2, T3 and T4) also improved embryo maturation and showed early germination even in maturation medium. It also accelerated normal embryo
germination frequency particularly in treatment with T2 and shortened ‘embryo—plantlet’ recovery time. Biochemical analyses revealed more proline, protein and amino acid with increasing
level of liquid overlaying as it improved embryo induction, development and faster germination. |
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