Inositol (1,4,5)trisphosphate-promoted Ca2+ release from microsomal fractions of rat liver |
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Authors: | A P Dawson R F Irvine |
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Institution: | 1. School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K.;2. Department of Biochemistry, ARC Institute of Animal Physiology, Babraham, Cambridge CB2 4AT, U.K. |
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Abstract: | Crude mitochondrial fractions containing a substantial amount of microsomes accumulate Ca2+ in the presence of ATP, ruthenium red and oligomycin. A proportion of this accumulated Ca2+ is released by the addition of low concentrations (ca. 1 microM) of inositol (1,4,5) trisphosphate . Under some conditions the release is transient, and evidence is presented which suggests that this is due to inhomogeneity in the vesicle population. (1,4,5)inositol trisphosphate -induced Ca2+ release can also be demonstrated, under appropriate experimental conditions, in a more purified microsomal fraction essentially free of mitochondria. |
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Keywords: | e r endoplasmic reticulum RSER rapidly sedimenting endoplasmic reticulum |
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