| 不同富集方法分离多环芳烃降解菌的比较研究 |
| |
| 引用本文： | 周宏伟,吴锦雅.不同富集方法分离多环芳烃降解菌的比较研究[J].现代生物医学进展,2007,7(9):1308-1309,1312. |
| |
| 作者姓名： | 周宏伟 吴锦雅 |
| |
| 作者单位： | 1. 南方医科大学,公共卫生与热带医学学院环境卫生学系,广东,广州,510515
2. 南方医科大学,科技处,广东,广州,510515 |
| |
| 摘 要： | 多环芳烃是一类普遍存在的环境污染物。本研究探讨了普通富集法,固定化富集法以及巴斯德消毒后富集法三种途径从相同红树林土壤中分离菲降解茵的差异。通过平板培养和变性梯度凝胶电泳两种方法分析分离结果。上述方法分别获得以鞘氨醇单胞茵、分枝杆菌以及红球茵为优势菌群的群落,表明分离方法对多环芳烃降解菌多样性的研究是一种重要的影响因素。
|
| 关 键 词： | 多环芳烃 富集法 鞘氨醇单胞菌 分枝杆菌 降解 |
| 文章编号： | 1673-6273（2007）09-1308-02 |
| 修稿时间： | 2007-06-062007-06-28 |
Comparison of Enrichment Methods for the Isolation of Polycyclic Aromatic Hydrocarbon-degrading Bacteria |
| |
| ZHOU Hong-wei,WU Jin-ya.Comparison of Enrichment Methods for the Isolation of Polycyclic Aromatic Hydrocarbon-degrading Bacteria[J].Progress in Modern Biomedicine,2007,7(9):1308-1309,1312. |
| |
| Authors: | ZHOU Hong-wei WU Jin-ya |
| |
| Institution: | 1 Department of enviornmental health, Institute of Public Health and Trop Medical, 2 Diviginal of Science and Technology, Southern Medical University, 510515 China |
| |
| Abstract: | Polycyclic aromatic hydrocarbons are a group of ubiquitous pollutants.The present study compared the isolation of phenanthrene-degrading bacteria from mangrove sediment by traditional,immobilized and pasteurized enrichment methods.Cultivation on agar plates and denaturant gradient gel electrophoresis data showed that the three methods resulted in the isolation of Sphingomonas,Mycobacterium and Rhodococcus strains,respectively,indicating that the enrichment method was critical for the study of diversity of polycydic aromatic hydrocarbon-degrading bacteria. |
| |
| Keywords: | Polycyclic aromatic hydrocarbon Enrichment Sphingomonas Mycobacterium Biodegradation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
