TOBACCO MOSAIC VIRUS IN CHLOROPLAST AND CYTOPLASM OF INFECTED TOBACCO LEAF

Chloroplasts containing Tobacco Mosaic Virus (TMV) were isolatedfrom TMV inoculated tobacco leaves, and TMV was extracted fromthem. The preparations from isolated chloroplasts of infectedleaves showed 30% increase in the optical density at 260 mµover those from healthy leaves. Most of infectivity was observedin chloroplast fraction 40 hr after inoculation. Thereafter,infectivity in the chloroplast fraction decreased and that ofcytoplasm increased with time. (Received April 6, 1964; )