6种抗真菌药物对皮肤癣菌体外抗真菌活性评价

目的评价6种抗真菌药物对皮肤癣菌体外抗真菌活性。方法采用CLSI推荐的M-38P方案对分离自足癣和体、股癣的皮肤癣菌进行联苯苄唑、硝酸舍他康唑、硝酸异康唑、盐酸布替萘芬、阿莫洛芬、利拉萘酯6种抗真菌药物敏感性测定。结果联苯苄唑MIC范围为0．03—4mg／L，MIC50为1mg／L，MIC90为2mg／L。硝酸舍他康唑分别为0．06—16mg／L、0．5mg／L和2mg／L。硝酸异康唑分别为0．03～2mg／L、0．25mg／L和0．5mg／L。盐酸布替萘芬分别为0．0025～0．04mg／L、0．01mg／L和0．02mg／L。阿莫罗芬分别为0．01～〉0．08mg／L、0．02mg／L和0．04mg／L。利拉萘酯分别为0．004—0．625mg／L、0．039mg／L和0．312mg／L。结论6种抗真菌药物对皮肤癣菌均有强的抗菌活性，由强到弱依次为布替萘芬、阿莫罗芬、利拉萘酯和咪唑类药物。

Evaluation of in vitro antifungal activities of six antifungal agents against dermatophytes

Objective To evaluate in vitro antifungal activities of six antifungal agents against dermatophytes. Methods Sensitivities of dermatophytes isolated from tinea pedis and tinea corporis ＆ cmris to bifonazole, sertaconazole nitrate, isoconazole nitrate, butenafine HCL, amorolfine,liranaftate were tested by broth microdilution method according to CLSI M-38P. Results The MICs MIC50 and MIC90 of butenafine HCL were 0. 002 5 - 0.04 mg/L,0.01 mg/L,0. 02 mg/L, respectively. Amorolfine 0.01 - 〉 0.08 mg/L, 0.02 mg/L and 0. 04 mg/L, respectively. Liranaftate 0. 004 -0. 625 mg/L, 0.039 mg/L and 0.312 mg/L, respectively. Isoconazole nitrate 0.03 -2 mg/L, 0.25 mg/L and 0. 5 mg/L, respectively. Sertaconazole nitrate 0.06 - 16 mg/L, 0.5 mg/L and 2 mg/L, respectively. Bifonazole 0.03 - 4 mg/L, 1 mg/L and 2 mg/L, respectively. Conclusions Our data suggested that six antifungal agents possess potent in vitro antifungal activities against dermatophytes,with butenafine HCL, amorpholine,liranaftate and azole antifungals in decreasing order.