Replication of wild-type and mutant human cytomegalovirus in life-extended human diploid fibroblasts |
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| Authors: | Bresnahan W A Hultman G E Shenk T |
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| Affiliation: | Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-1014, USA. wbresnahan@molbio.princeton.edu |
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| Abstract: | A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity. |
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