DTNB对人肌肌酸激酶不可逆抑制作用的研究

本文研究了DTNB对人肌肌酸激酶的不可逆抑制作用.研究结果表明,与兔肌肌酸激酶不同,人肌肌酸激酶分子中有四个可反应SH.用邹承鲁作图法定量处理结果表明,在这四个可反应的SH中,有一个快反应SH,两个慢反应SH且这两个慢反应SH是酶的必需基团,此外还有一个反应很慢的SH.用邹承鲁提出的在抑制剂存在条件下,酶活性不可逆改变动力学方法,测定了一系列动力学常数,并对DTNB的作用机制进行了探讨.

STUDIES ON THE IRREVERSIBLE INHIBITION OF CREATINE KINASE FROM HUMAN MUSCLE BY DTNB

The irreversible inhibition of creatine kinase from human muscle during modification with DTNB has been studied. It has been found that four SH groups per enzyme molecule, which is different from rabbit muscle CK, could be modified. The result handled by Tsou's plot suggests that one of the four reactive SH groups is fast reactive and nonessential for activity, two of them are slow and essential, and another is very slow and nonessential. A series of kinetic constants have been measured, with the method of kinetics of irreversible modification of enzyme activity in the presence of inhibitor put forward by Tsou, and the acting mechanism of DTNB has also been discussed.