Genetic Structure and Low-genetic Diversity Suggesting the Necessity for Conservation of the Chinese Longsnout Catfish, Leiocassis longirostris (Pisces: Bagriidae) |
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| Authors: | Zhongwei Wang Jianfeng Zhou Yuzhen Ye Qiwei Wei Qingjiang Wu |
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| Institution: | (1) State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072 Wuhan, P.R. China;(2) Graduate School of Chinese Academy of Sciences, Beijing, China;(3) Ministry of Agriculture, The Key Lab of Freshwater Fish Germplasm and Biotechnology, P.R. China;(4) Yangtze River Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 434000 Jingzhou, Hubei, P.R. China |
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| Abstract: | Synopsis Genetic variation and phylogenetic relationship of Leiocassis longirostris populations from the Yangtze River were investigated at mitochondrial DNA level. The samples were collected from the upstream
and mid-downstream of the Yangtze River. Three mitochondrial DNA fragments, ND5/6, cytochrome b (Cyt b) and control region (D-loop), were amplified and then digested by 10 restriction endonucleases. Twenty-three D-loop fragments
randomly selected were sequenced. Digestion patterns of ND5/6 by AluI and HaeIII, D-loop by HinfI and RsaI, and Cyt b by HaeIII were polymorphic. Ten and eighteen haplotypes were obtained from RFLP data and sequence data, respectively. The individuals
from upstream and mid-downstream of the Yangtze River were apparently divided into two groups. The average genetic distance
was 0.008 and 0.010 according to the two data. Low diversities and decreasing abundance indicated that Leiocassis longirostris may be in severe danger and reasonable measures of fishery management should be taken. |
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| Keywords: | Leiocassis longirostris mitochondrial DNA genetic diversity PCR– RFLP |
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