硝酸镧和兰科菌根真菌互作对石斛生长和有效成分积累的影响

利用盆栽的方式研究了不同硝酸镧水平下接种OM真菌对石斛生长、根系活力、N、P、K等营养元素吸收以及有效成分积累的影响,并分析了根际硝酸还原酶和磷酸酶活性变化。结果表明,添加适量的硝酸镧有利于OM真菌侵染和菌根发育,促进石斛生长,包括地下和地上的生物量。在接种OM真菌时,同时添加5.0 mg·L-1的硝酸镧,石斛的生物量达到最大,为对照组的4.76倍。在适量的硝酸镧水平下接种OM真菌还可以提高根系活力,并促进N、P和K的吸收和利用,提高根际硝酸还原酶和磷酸酶的活性;显著提高蛋白质、多糖以及石斛碱的含量。硝酸镧与OM真菌互作能促进石斛生长和有效成分的积累,提高其药用品质。

Effects of Lanthanum Nitrate and Orchid Mycorrhizal Fungi on the Growth and Active Component Accumulation in Dendrobium nobile

The effects of lanthanum nitrate and inoculation of OM fungus on the growth, root activity, uptake of nutrients ofDendrobium nobile and the active component accumulation were investigated using a pot experiment. The activities of nitrate reductase and phosphatase were also analyzed. The results indicated that appropriate concentration of lanthanum nitrate promoted the development of mycorrhiza. OM fungus promoted the growth of D. nobile, including underground and aboveground biomass. Moreover, D. nobile inoculated with OM fun- gus and applied with lanthanum nitrate 5.0 mg.L^-1 had the largest biomass which was of 4.76-fold compared to control. Both lanthanum nitrate application and inoculation with OM fungus increased root activity and nutrient absorption. The activities of nitrate reductase and phosphatase also increased. The proteins, polysaccharides and dendrobine in levels increased significantly. The inoculation with OM fungus and application with lanthanum nitrate can promote the growth ofD. nobile and the content of active components.